gangliosides  (Avanti Polar)

Journal: ACS Omega

Article Title: Single-Virus Lipid-Mixing Study of Sendai Virus Provides Insight into Fusion Mechanism

doi: 10.1021/acsomega.5c07645

Figure Lengend Snippet: Cumulative distribution function (CDF) of SeV lipid mixing wait times follows a single exponential model. The distribution of wait times compiled from single-virus lipid mixing measurements is shown as a CDF (blue circles), and modeled by a single exponential function with time lag (black line). The extent of lipid mixing was calculated as the fraction of virions that underwent lipid mixing during 90 min of observation ( N = 159/3829). Lipid composition of target liposomes = 2% GQ1b, 20% DOPE, 30% cholesterol, 1% 16:0 biotinyl PE, 0.05% OG-DHPE, and 46.95% POPC.

Article Snippet: Dioleoylphosphatidylethanolamine (DOPE), palmitoyloleoylphosphatidylcholine (POPC), cholesterol (Chol), 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine-N-biotinyl (biotinyl PE), and ganglioside receptors GD1a and GQ1b were purchased from Avanti Polar Lipids (Alabaster, AL, USA).

Techniques: Virus, Liposomes

Journal: ACS Omega

Article Title: Single-Virus Lipid-Mixing Study of Sendai Virus Provides Insight into Fusion Mechanism

doi: 10.1021/acsomega.5c07645

Figure Lengend Snippet: Trypsin treatment increases lipid mixing extent, with little change to CDF. Single-virus lipid mixing experiments were conducted with SeV that had been pretreated with various concentrations of TPCK-trypsin (0–1000 μg/mL). (A) shows the distributions of observed wait times. (B) shows the extents of lipid mixing, calculated as the fraction of observed virions that underwent lipid mixing during 90 min of observation. N virus : 0 μg/mL = 159/3829, 10 μg/mL = 52/1188, 100 μg/mL = 443/5959, 1000 μg/mL = 162/1938. Values shown are the mean ± 95% confidence interval determined by bootstrap resampling (NumBootstraps = 10,000). Extents at 100 μg/mL and 1000 μg/mL were statistically different from 0 μg/mL and 10 μg/mL with p < 0.0002 determined by bootstrap. All other extent comparisons were not significant. Lipid composition of target liposomes = 2% GQ1b, 20% DOPE, 30% cholesterol, 1% 16:0 biotinyl PE, 0.05% OG-DHPE, and 46.95% POPC.

Article Snippet: Dioleoylphosphatidylethanolamine (DOPE), palmitoyloleoylphosphatidylcholine (POPC), cholesterol (Chol), 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine-N-biotinyl (biotinyl PE), and ganglioside receptors GD1a and GQ1b were purchased from Avanti Polar Lipids (Alabaster, AL, USA).

Techniques: Virus, Liposomes

Journal: ACS Omega

Article Title: Single-Virus Lipid-Mixing Study of Sendai Virus Provides Insight into Fusion Mechanism

doi: 10.1021/acsomega.5c07645

Figure Lengend Snippet: Influence of receptor identity on SeV lipid mixing. Single-virus lipid mixing experiments were conducted to target liposomes with different receptors: either 2% GQ1b or 2% GD1a. SeV had been pretreated 100 μg/mL TPCK-trypsin. (A) shows the distributions of observed wait times. (B) shows the extent of lipid mixing for each receptor, calculated as the fraction of observed virions that underwent lipid mixing during the experiment. N virus for extents were: 2% GQ1b = 475/5959, 2% GD1a = 154/2404. Values shown are the mean ± 95% confidence interval determined by bootstrap resampling (NumBootstraps = 10,000). Extents between the 2 conditions were statistically different with p < 0.02 determined by bootstrap. Lipid composition of target liposomes = 2% GQ1b or GD1a, 20% DOPE, 30% cholesterol, 1% 16:0 biotinyl PE, 0.05% OG-DHPE, and 46.95% POPC.

Article Snippet: Dioleoylphosphatidylethanolamine (DOPE), palmitoyloleoylphosphatidylcholine (POPC), cholesterol (Chol), 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine-N-biotinyl (biotinyl PE), and ganglioside receptors GD1a and GQ1b were purchased from Avanti Polar Lipids (Alabaster, AL, USA).

Techniques: Virus, Liposomes